Selleck Chemicals/Temsiromus（CCI-779）/S1044-免疫在线蚂蚁淘旗下平台

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商品详细Selleck Chemicals/Temsiromus（CCI-779）/S1044

Selleck Chemicals/Temsiromus（CCI-779）/S1044

商品编号： S1044

品牌： Selleck Chemicals

市场价： ¥17400.00

美元价： 10440.00

产地：美国(厂家直采)

公司：

产品分类：聚合物

公司分类： juhewu

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

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商品介绍

Description

Temsirolimus (CCI-779, NSC 683864) is a specific mTOR inhibitor with IC50 of 1.76 μM in a cell-free assay. Temsirolimus induces autophagy and apoptosis.

Targets

mTOR ^[1](Cell-free assay)

1.76 μM

In vitro

In the absence of FKBP12, Temsirolimus potently inhibits mTOR kinase activity with IC50 of 1.76 μM, similar to that of rapamycin with IC50 of 1.74 μM. Temsirolimus treatment at nanomolar concentrations (10 nM to <5 μm)="" displays="" a="" modest="" and="" selective="" antiproliferative="" activity="" via="" fkbp12-dependent="" mechanism,="" but="" can="" completely="" inhibit="" the="" proliferation="" of="" a="" broad="" panel="" of="" tumor="" cells="" at="" low="" micromolar="" concentrations="" (5-15="" μm),="" involving="" fkbp12-independent="" suppression="" of="" mtor="" signaling.="" temsirolimus="" treatment="" at="" micromolar="" but="" not="" nanomolar="" concentrations="" (20="" μm)="" causes="" a="" marked="" decline="" in="" global="" protein="" synthesis="" and="" disassembly="" of="" polyribosomes,="" accompanied="" by="" rapid="" increase="" in="" the="" phosphorylation="" of="" translation="" elongation="" factor="" eef2="" and="" the="" translation="" initiation="" factor="" eif2a.="">^[1] Temsirolimus inhibits the phosphorylation of ribosomal protein S6, more potently in PTEN-positive DU145 cells than in PTEN-negative PC-3 cells, and inhibits cell growth and clonogenic survival of both cells in a concentration-dependent manner. ^[2] Temsirolimus (100 ng/mL) potently inhibits proliferation and induces apoptosis in primary human lymphoblastic leukemia (ALL) cells. ^[3]

Cell Data

Cell Lines	Assay Type	Incubation Time	Activity Description	PMID
human HSC-4 cell	NIC4botIem:5dHigbY5pcWKrdHnvckBie3OjeR?=		NYDkdW9TUW6qaXLpeIlwdiCxZjDoeY1idiCKU1OtOEBkWyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvOTF\|IH7N	M1jzSXNCVkeHUh?=
human L-363 cell	MljYS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl?		MUnJcohq[mm2aX;uJI9nKGi3bXHuJGwuOzZ\|IHPlcIwh3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO\|YYmsJGlEPTB;MD6yOFIhdk1?	NGLJcZRUSU6JRWK=
human Ramos-2G6-4C10 cell	NGjR[I1Iem:5dHigbY5pcWKrdHnvckBie3OjeR?=		MmjLTY5pcWKrdHnvckBwiCqdX3hckBT[W2xcz2yS\|YuPENzMDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG\|c3H5MEBKSzVyPUCuN\|QzKG6P	M2rXWHNCVkeHUh?=
human SBC-1 cell	NUPKRW1jT3Kxd4ToJIlvcGmkaYTpc44h[XO\|YYm=		NV7CZnNWUW6qaXLpeIlwdiCxZjDoeY1idiCVQlOtNUBkWyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvPDR2IH7N	NYj4PFlCW0GQR1XS
human MHH-PREB-1 cell	MkLES5Jwf3SqIHnubIljcXSrb36gZZN{[Xl?		M3TKNWlvcGmkaYTpc44hd2ZiaIXtZY4hVUiKLWDSSWIuOSClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR\|UxRTBwNEm1JI5O	M{Hj[XNCVkeHUh?=
human LNCAP cells	M4PpXnBzd2yrZnXyZZRqd25iYYPzZZk>	MVyzJIRigXN?	NIDSc5dCdnSrcILvcIlnXKjdHn2[UBi[3Srdnn0fUBi2GrboP0JIh2dWGwIFzOR2FRKGOnbHzzJIFnfGW{IEOg[IF6eyCkeTDNWHMh[XO\|YYmsJGlEPTB;MD61JI5O	MlfrNlE1Ozh3N{m=
human HH cell	MnHJS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl?		NF3qRYNKdmirYnn0bY9vKG:oIHj1cYFvKEiKIHPlcIwh3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO\|YYmsJGlEPTB;MD62OlQhdk1?	MVfTRW5ITVJ?
human TYK-nu cell	MkPLS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl?		MlTPTY5pcWKrdHnvckBwiCqdX3hckBVYUtvboWgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlc4QCCwTR?=	NGT6[ZhUSU6JRWK=
human H4 cell	NUizZ3ByT3Kxd4ToJIlvcGmkaYTpc44h[XO\|YYm=		M1PrPWlvcGmkaYTpc44hd2ZiaIXtZY4hUDRiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1zLkC3JI5O	MVPTRW5ITVJ?
human K5 cell	NFvZXHVIem:5dHigbY5pcWKrdHnvckBie3OjeR?=		MX7Jcohq[mm2aX;uJI9nKGi3bXHuJGs2KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OS5\|MzDuUS=>	MkX0V2FPT0WU
human PA-1 cell	MYnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>?		MWjJcohq[mm2aX;uJI9nKGi3bXHuJHBCNTFiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1zLkS3JI5O	M{XsUHNCVkeHUh?=
human SK-NEP-1 cell	MULHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>?		NGjBXndKdmirYnn0bY9vKG:oIHj1cYFvKFONLV7FVE0yKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OS52OTDuUS=>	MnnMV2FPT0WU
human 697 cell	NXX6VnZTT3Kxd4ToJIlvcGmkaYTpc44h[XO\|YYm=		NUfNVINLUW6qaXLpeIlwdiCxZjDoeY1idiB4OUegZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xMlchdk1?	NHfvcIZUSU6JRWK=
human CTB-1 cell	MUHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>?		MmO1TY5pcWKrdHnvckBwiCqdX3hckBEXEJvMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG\|c3H5MEBKSzVyPUKuPVMhdk1?	NYjKb4JXW0GQR1XS
human DB cell	MlfwS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl?		NWP0V\|dGUW6qaXLpeIlwdiCxZjDoeY1idiCGQjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG\|c3H5MEBKSzVyPUOuNVIhdk1?	NEKxPVNUSU6JRWK=
human MLMA cell	NUO0N5NrT3Kxd4ToJIlvcGmkaYTpc44h[XO\|YYm=		NVfJR5A{UW6qaXLpeIlwdiCxZjDoeY1idiCPTF3BJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9N{4{OSCwTR?=	MoTpV2FPT0WU
human GAMG cell	NGDpe4tIem:5dHigbY5pcWKrdHnvckBie3OjeR?=		MmfqTY5pcWKrdHnvckBwiCqdX3hckBISU2JIHPlcIwh3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO\|YYmsJGlEPTB;Mz64NkBvVQ>?	NVrrbY9NW0GQR1XS
human JVM-3 cell	MV3Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>?		M3LMdWlvcGmkaYTpc44hd2ZiaIXtZY4hUl[PLUOgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2zMlk2KG6P	MmjHV2FPT0WU
human LAMA-84 cell	NFzaWoxIem:5dHigbY5pcWKrdHnvckBie3OjeR?=		M2TNR2lvcGmkaYTpc44hd2ZiaIXtZY4hVEGPQT24OEBkWyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVQvODNibl2=	NVLGbIVSW0GQR1XS
human RPMI-8226 cell	NHS3OopIem:5dHigbY5pcWKrdHnvckBie3OjeR?=		Mn2zTY5pcWKrdHnvckBwiCqdX3hckBTWE2LLUiyNlYh[2WubDDndo94fGhiaX6gZUBkWyuIIpZYJqdGm2eTDhd5NigSxiSVO1NF01NjF3IH7N	NVKV2trW0GQR1XS
human MOLT-4 cell	NHvvWVVIem:5dHigbY5pcWKrdHnvckBie3OjeR?=		M2nIPGlvcGmkaYTpc44hd2ZiaIXtZY4hVU:OVD20JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OE4{OSCwTR?=	NIfsZ3hUSU6JRWK=
human CAMA-1 cell	NGL5dnRIem:5dHigbY5pcWKrdHnvckBie3OjeR?=		MXHJcohq[mm2aX;uJI9nKGi3bXHuJGNCVUFvMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG\|c3H5MEBKSzVyPUSuO\|chdk1?	MmDwV2FPT0WU
human NCI-SNU-1 cell	NUe0cHNHT3Kxd4ToJIlvcGmkaYTpc44h[XO\|YYm=		NF1R3ZKdmirYnn0bY9vKG:oIHj1cYFvKE6FST3TUnUuOSClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR\|UxRTRwOEGgcm0>	NFG0WpNUSU6JRWK=
human SW780 cell	NVr5TZp3T3Kxd4ToJIlvcGmkaYTpc44h[XO\|YYm=		NVnpO4FqUW6qaXLpeIlwdiCxZjDoeY1idiCVV{e4NEBkWyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVQvQDNibl2=	MVrTRW5ITVJ?
human H9 cell	NED3eo5Iem:5dHigbY5pcWKrdHnvckBie3OjeR?=		NF7wbpVKdmirYnn0bY9vKG:oIHj1cYFvKEh7IHPlcIwh3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO\|YYmsJGlEPTB;ND64PEBvVQ>?	NIrKW\|FUSU6JRWK=
human BE-13 cell	M3;Tc2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7		NH;De5hKdmirYnn0bY9vKG:oIHj1cYFvKEKHLUGzJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OE46OSCwTR?=	MXLTRW5ITVJ?
human ES8 cell	M2CzN2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7		MVfJcohq[mm2aX;uJI9nKGi3bXHuJGVUQCClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR\|UxRTVwMkegcm0>	NFLi[XVUSU6JRWK=
human DEL cell	MkD3S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl?		MXvJcohq[mm2aX;uJI9nKGi3bXHuJGRGVCClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR\|UxRTVwNE[gcm0>	NFz6epNUSU6JRWK=
human QIMR-WIL cell	NIj0NodIem:5dHigbY5pcWKrdHnvckBie3OjeR?=		NYMUphxUW6qaXLpeIlwdiCxZjDoeY1idiCTSV3SMXdKVCClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR\|UxRTVwN{Ggcm0>	NUHmdIJzW0GQR1XS
human CGTH-W-1 cell	Mn7pS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl?		MUTJcohq[mm2aX;uJI9nKGi3bXHuJGNIXEhvVz2xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OU44PSCwTR?=	M{HYO3NCVkeHUh?=
human CHL-1 cell	MlPS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl?		NVPG[WxzUW6qaXLpeIlwdiCxZjDoeY1idiCFSFytNUBkWyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVUvQDZibl2=	M4TFW3NCVkeHUh?=
human A2780 cell	NVvU[\|B6T3Kxd4ToJIlvcGmkaYTpc44h[XO\|YYm=		MWJcohq[mm2aX;uJI9nKGi3bXHuJGEzPzhyIHPlcIwh3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO\|YYmsJGlEPTB;NT64PEBvVQ>?	NUfI[FV{W0GQR1XS
human VA-ES-BJ cell	MoHKS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl?		NULtRXhNUW6qaXLpeIlwdiCxZjDoeY1idiCYQT3FV{1DUiClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR\|UxRTVwOTDuUS=>	NEPTWWNUSU6JRWK=
human BB65-RCC cell	MYHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>?		MmW4TY5pcWKrdHnvckBwiCqdX3hckBDSjZ3LWLDR{BkWyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVchdk1?	NYkSmlGW0GQR1XS
human D-566MG cell	M1rhTGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7		M1jRSGlvcGmkaYTpc44hd2ZiaIXtZY4hTC13NkNS{BkWyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVcvOTZibl2=	MVvTRW5ITVJ?
human MDA468 cells	NUjDOFlCWHKxbHnm[ZJifGmxbjDhd5NigQ>?	NXLCe2JGOyCmYYnz	M3vrUWFvfGmycn;sbYZmemG2aYlJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTVTBOFY5KGOnbHzzJIFnfGW{IEOg[IF6eyCkeTDNWHMh[XO\|YYmsJGlEPTB;ODDuUS=>	M4r6R\|IyPDN6NUe5
human HO-1-N-1 cell	M1jhOWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7		M3PNOmlvcGmkaYTpc44hd2ZiaIXtZY4hUE9vMT3OMVEh[2WubDDndo94fGhiaX6gZUBkWyuIIpZYJqdGm2eTDhd5Nige,:jDDJR\|UxRThwMjDuUS=>	NYPFXFJUW0GQR1XS
human RS4-11 cell	NUXDOlhZT3Kxd4ToJIlvcGmkaYTpc44h[XO\|YYm=		MYTJcohq[mm2aX;uJI9nKGi3bXHuJHJUPC1zMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG\|c3H5MEBKSzVyPUiuOFUhdk1?	M1Hwe3NCVkeHUh?=
human PC-3 cell	NV7Fc3k2T3Kxd4ToJIlvcGmkaYTpc44h[XO\|YYm=		MYnJcohq[mm2aX;uJI9nKGi3bXHuJHBENTNiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD17LkG0JI5O	MVXTRW5ITVJ?
human NB69 cell	MkfmS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl?		M17wNGlvcGmkaYTpc44hd2ZiaIXtZY4hVkJ4OTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG\|c3H5MEBKSzVyPUmuOFYhdk1?	NXBWJZDW0GQR1XS
human HGC-27 cell	MWXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>?		NEXhcY5KdmirYnn0bY9vKG:oIHj1cYFvKEiJQz2yO{BkWyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVkvPiCwTR?=	Mkm2V2FPT0WU
human NCI-H720 cell	NUfR[Yk1T3Kxd4ToJIlvcGmkaYTpc44h[XO\|YYm=		M3Hs[2lvcGmkaYTpc44hd2ZiaIXtZY4hVkOLLVi3NlAh[2WubDDndo94fGhiaX6gZUBkWyuIIpZYJqdGm2eTDhd5NigSxiSVO1NF06NjZ{IH7N	MVLTRW5ITVJ?
human NCI-H292 cell	MlPhS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl?		NUf2dlh4UW6qaXLpeIlwdiCxZjDoeY1idiCQQ1mtTFI6OiClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR\|UxRTlwOESgcm0>	NFHHSZZUSU6JRWK=
human A3-KAW cell	MnLZS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl?		MYLJcohq[mm2aX;uJI9nKGi3bXHuJGE{NUuDVzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG\|c3H5MEBKSzVyPUGwMlQ{KG6P	NH7tfWlUSU6JRWK=
human DU-4475 cell	M1WwOWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7		MUPJcohq[mm2aX;uJI9nKGi3bXHuJGRWNTR2N{WgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xNU44OyCwTR?=	NEfXTGlUSU6JRWK=
human HuH-7 cell	NIPqOHNIem:5dHigbY5pcWKrdHnvckBie3OjeR?=		MlLTTY5pcWKrdHnvckBwiCqdX3hckBJfUhvNzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG\|c3H5MEBKSzVyPUGyMlMzKG6P	MoL5V2FPT0WU
human J-RT3-T3-5 cell	NFP0WI9Iem:5dHigbY5pcWKrdHnvckBie3OjeR?=		MX7Jcohq[mm2aX;uJI9nKGi3bXHuJGouWlR\|LWSzMVUh[2WubDDndo94fGhiaX6gZUBkWyuIIpZYJqdGm2eTDhd5NigSxiSVO1NF0yOi5\|ODDuUS=>	NF;RVIZUSU6JRWK=
human VM-CUB-1 cell	NF;sOWRIem:5dHigbY5pcWKrdHnvckBie3OjeR?=		NHXXV45KdmirYnn0bY9vKG:oIHj1cYFvKF[PLVPVRk0yKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OTJwNkegcm0>	M1y1VnNCVkeHUh?=
human MOLT-16 cell	M{XkNmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7		NEHEToZKdmirYnn0bY9vKG:oIHj1cYFvKE2RTGStNVYh[2WubDDndo94fGhiaX6gZUBkWyuIIpZYJqdGm2eTDhd5NigSxiSVO1NF0yOi56NDDuUS=>	MmPHV2FPT0WU
human KG-1 cell	M36xOGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7		NVfFUnl[UW6qaXLpeIlwdiCxZjDoeY1idiCNRz2xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVIvQSCwTR?=	MVXTRW5ITVJ?
human P12-ICHIKAWA cell	NFS5bFBIem:5dHigbY5pcWKrdHnvckBie3OjeR?=		M37lRWlvcGmkaYTpc44hd2ZiaIXtZY4hWDF{LVnDTGlMSVeDIHPlcIwh3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO\|YYmsJGlEPTB;MUOuNFEhdk1?	M1vJc3NCVkeHUh?=
human ACN cell	NGHXZ4pIem:5dHigbY5pcWKrdHnvckBie3OjeR?=		MmHnTY5pcWKrdHnvckBwiCqdX3hckBCS05iY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1zMz6wNkBvVQ>?	NWfFR4JPW0GQR1XS
human COLO-684 cell	M{ Vmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7		MVrJcohq[mm2aX;uJI9nKGi3bXHuJGNQVE9vNki0JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVMvOzFibl2=	Mnf3V2FPT0WU
human T-24 cell	MlzBS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl?		NF;E[ZdKdmirYnn0bY9vKG:oIHj1cYFvKFRvMkSgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xN{45PiCwTR?=	M{6PXNCVkeHUh?=
human AGS cell	MYLHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>?		M{nqcGlvcGmkaYTpc44hd2ZiaIXtZY4hSUeVIHPlcIwh3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO\|YYmsJGlEPTB;MUSuNVEhdk1?	NWLpU\|hkW0GQR1XS
human EW-13 cell	MljMS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl?		NFnXbo5KdmirYnn0bY9vKG:oIHj1cYFvKEWZLUGzJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVQvPTRibl2=	M3TLSXNCVkeHUh?=
human SW962 cell	NWTwUGU1T3Kxd4ToJIlvcGmkaYTpc44h[XO\|YYm=		MX;Jcohq[mm2aX;uJI9nKGi3bXHuJHNYQTZ{IHPlcIwh3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO\|YYmsJGlEPTB;MUWuOVkhdk1?	NXHRfGcxW0GQR1XS
human EW-11 cell	Mof6S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl?		M2rGT2lvcGmkaYTpc44hd2ZiaIXtZY4hTVdvMUGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xOU43KG6P	M4jPcXNCVkeHUh?=
human RXF393 cell	M3zCWWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7		M1m1dmlvcGmkaYTpc44hd2ZiaIXtZY4hWliIM{mzJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVUvPjZibl2=	MmPyV2FPT0WU
human EoL-1-cell cell	M{XOTGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7		MlXPTY5pcWKrdHnvckBwiCqdX3hckBGd0xvMT3j[YxtKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OTZwMTDuUS=>	NYfPeZdEW0GQR1XS

... Click to View More Cell Line Experimental Data

Assay

Methods	Test Index	PMID
Western blot	p-mTOR / mTOR / p-S6 / S6 / p-4E-BP1 / 4E-BP1 ; PubMed: 23291985Cancer Biol Ther The mTOR inhibitor temsirolimus inhibits the activation of mTOR and its downstream molecules in esophageal cancer cells. Three esophageal cancer cell lines (TE-1, TE-8, and TE-10) were treated with different concentrations of temsirolimus (0–1000nM) and western blot was performed with appropriate antibodies to detect the expression of mTOR and its downstream effectors. β-actin was served as an internal control. p-rS6 / rS6 / p-AKT /AKT ; PubMed: 22039466PLoS One C,D, Phosphorylation of rS6 (P-rS6) and Akt (P-Akt T308 and P-Akt S473) after treatment with indicated doses of temsirolimus for 72 hrs in temsirolimus-sensitive (C) or temsirolimus-resistant (D) cells was determined by Western blotting. Expression of total rS6 and Akt protein serves as loading controls.	23291985 22039466
Immunofluorescence	NRF2; PubMed: 22848625PLoS One Ectopic expression of Nrf2 (red) and nuclear staining with Dapi (blue) after a 6 hour drug incubation. Yellow numbers represent the percent of cells with nuclear Nrf2 +/− the standard deviation. Scale bar = 10 µM.	22848625
Growth inhibition assay	Cell growth (HGC-3 cells) ; PubMed: 30866995J Exp Clin Cancer Res Effect of temsirolimus on the growth of HGC-3 and -20 cells in primary culture. Cell viability ; PubMed: 22039466PLoS One A,B, Endometrial cancer cells were treated with increasing doses of temsirolimus for 72 hrs. Results are separated by (A) sensitivity or (B) resistance as determined by cell viability.	30866995 22039466

In vivo

In the NOD/SCID xenograft models with human ALL, Temsirolimus treatment at 10 mg/kg/day produces a decrease in peripheral blood blasts and in splenomegaly ^[3] Administration of Temsirolimus (20 mg/kg i.p. 5 days/week) significantly delays the growth of DAOY xenografts by 160% after 1 week and 240% after 2 weeks, compared with controls. Single high-dose of Temsirolimus (100 mg/kg i.p) treatment induces 37% regression of tumor volume within 1 week. Temsirolimus treatment for 2 weeks also delays the growth of rapamycin-resistant U251 xenografts by 148%. ^[4] Inhibition of mTOR by Temsirolimus improves performance on four different behavioral tasks and decreases aggregate formation in a mouse model of Huntington disease. ^[5] Administration of Temsirolimus induces significant dose-dependent, antitumor responses against subcutaneous growth of 8226, OPM-2, and U266 xenografts with ED50 of 20 mg/kg and 2 mg/kg for 8226 and OPM-2, respectively, which are associated with inhibited proliferation and angiogenesis, induction of apoptosis, and reduction in tumor cell size. ^[6]

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Apoptosis Neuronal Signaling Epigenetics MAPK 血管生成泛素 NF-κ；B Metabolism GPCR&；G蛋白

JAK/统计 Stem Cells & Wnt PI3K/Akt/mTOR公司 Cell Cycle 蛋白酪氨酸激酶

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