Ammonium pyrrolidine dithiocarbamate (PDTC) is a potent nuclear factor-κB (NF-κB) inhibitor that inhibits IκB phosphorylation, blocks NF-κB translocation to the nucleus and reduces the expression of downstream cytokines.
Targets
NF-κB [1]()
In vitro
Ammonium pyrrolidine dithiocarbamate (PDTC) can inhibit the role of NF-κB by inhibiting IκB phosphorylation, thus blocking NF-κB translocation to the nucleus, reducing the expression of downstream cytokines[1].
Assay
Methods
Test Index
PMID
Western blot
p-p65 / p65 / p50 ;
PubMed: 29956738
Following treatment with EMCL at the indicated doses and PDTC (30µM) for 24 h, cytoplasmic and nuclear extracts were prepared for the western blot analysis of p-p65, p65, and p50.
p-IKKβ / IKKβ / p-IκBα / IκBα ;
PubMed: 29956738
Following treatment with EMCL at the indicated doses and PDTC (30µM) for 24 h, the protein expression levels of IKKβ, p-IKKβ, IκBα, and p-IκBα were analyzed by western blot analysis.
CCR2 / CCR5 / CXCR4 ;
PubMed: 10716998
Dose-response (Left) and time course (Right) of the effect of PDTC on CCR2, CCR5, and CXCR4 mRNA expression. For the dose–response analysis, total RNA was purified from monocytes incubated for 4 h with PDTC at the indicated doses. In kinetic analysis, the total RNA was purified from fresh human monocytes incubated with 50 μM PDTC for the indicated times. Results inAare representative of three separate experiments.
29956738 10716998
Immunofluorescence
p65 / p50;
PubMed: 29956738
Following treatment with EMCL at the indicated doses and PDTC (30µM) for 24 h, the subcellular localizations of p50 and p65 in 786-0 cells were examined by confocal microscopy analysis(magnification, ×630).
